应用多种单克隆抗体联合检测大肠癌的早期诊断探索

A Research on Combined Detection in Early Diagnosis of Colon Cancer by Means of Various Monoclonal Antibodies

应用４种单克隆抗体、通过免疫组化Ｓ－Ｐ法，对大肠癌、大肠腺癌、癌旁粘膜和正常大肠粘膜进行标志．７２例大肠癌组织中阳性表达率分别为ＳＣ１２Ａ８７．５％；ＳＣ１６Ａ７６．３％；ＣＳＡ９１．６６％；ＣＥＡ９５．８％，与正常大肠粘膜阳性表达１３．３％比较，均有非常显著差异．２７例脉瘤组织中阳性表达率分别为ＳＣ１２Ａ５９．２５％；ＳＣ１６Ａ５９．２５％：ＣＳＡ７０．３７％；ＣＥＡ７０．３７％；和正常大肠粘膜比较有显著差异（Ｐ＜０．０５）；和大肠癌比较、除ＳＣ１６Ａ无显著差异（Ｐ＞０．０５）外，其它三种均有高度差异（Ｐ＜０．０１），对排除癌有一定意义．４９例癌旁粘膜阳性表达率分别为：ＳＣ１２Ａ５７．１％；ＳＣ１６Ａ４６．９％；ＣＳＡ６７．３％；ＣＥＡ５１％阳性细胞分布虽然主要在腺腔内缘．范围较局限．但这么高的抗原表达，支持了Ｆｉｌｉｐｅ等的“移行粘膜”

Four monoclonal antibodies are employed to mark colon cancer, adenocarcinoma of the colon, premalignant mucosa and normal colon mucosa by the histochemical S─P method. Among the 72 cases of colon cancer, the positive rates are SC12A 87. 5%, SC16A 76. 3 %, CSA 91. 66 % and CEA 95. 8%, respectively. They have significant difference when compared with that of normal colon mucosa(13. 3 % ).For other 27 cases of adenocarcinoma the positive rates are SC12A 59. 25%, SC16A 59.25%, CSA 70. 37% and CEA 70. 37%, respectively. Compared with the normal colon mucosa, there is significant difference (P<0 .05). Except that SC16A has showed no significant difference (P > 0. 05 ), the other three have showed significant difference when compared with that of colon cancer (P<0. 01 ). Thus the result would be valuable in diagnosing cancer.Besides, for 49 premalignant mucosa cases the positive rates are SC12A 57. 1 %, SC16A 46. 9 %, CSA67. 3 % and CEA 51 %, respectively. Though the positive cells concentrated on the inner fringe of the glandular cavity, this high expression of antigens supports the 'Transitional mucosa' theory given by Filipe et al.