从鳕鱼碎肉中提取水解蛋白

PREPARATION OF PROTEIN HYDROLYSATE FROM FISH OFFAL OF POLLOCK (THERAGRA CHALCOGRAMMA)

用蛋白酶水解法从鳕鱼加工废弃物──鳕鱼碎肉中提取水解蛋白。鳕鱼碎肉经枯草杆菌AS1．398中性蛋白酶240IU／ml，pH7．0，50℃下水解2h；再经黄曲霉IFFI2342（蛋白酶活力2000IU／g）1．5％（W／v），50℃，pH6．0下水解6h以脱苦味。水解液经真空浓缩后喷雾干燥得水解蛋白粉。该制品易溶于水，无苦味，有海鲜味。其氨基酸组成合理，是优良的蛋白质制品。

A fish protein hydrolysate was prepared from fish offal, the processing waste of pollock (Theragra chalcogramma), by hydrolysis with proteolytic enzymes. The offal was first hydrolyzed with 240IU/ml AS 1.398 neutral proteinase at 50℃, pH 7.0, for 2h, and then, in order to eliminate the bitter taste, the hydrolysate was further hydrolyzed with 1.5% (w/w) IFF12342 (Proteinase activity was 2000IU/g)at 50℃, pH6.0 for 6h. The end product gained by vacuum concentration and spray dry from the hydrolysate was soluble in water without bitter taste but with the flavor of seafood, and the amino acids composition was favorable. It is a kind of high quality protein product.