离子束注入抗生素产生菌诱变效应的研究

A study on mutagenic effects of antibiotic-producers by ion implantation

选用不同剂量的矿Ｎ＋和Ｃ＋注入核糖霉素产生菌、卡那霉素和卡那霉素拉株产生菌，研究其诱变效果。实验结果表明：菌种死亡率随剂量的增加而增加，形态变异比较明显；效价单位提高：Ｎ＋处理核糖霉素产生菌为１０％－２５％以上，卡那霉素和卡那霉素抗株产生苗分别为５．２％－１２．１％和２．１％－１２．７５％；Ｃ＋处理核糖霉素产生菌为１０％－１６．９％，卡那霉素抗株产生菌为５％－２０％，卡那霉素产生菌为１．０５％－３．０８％．用Ｎ＋处理生产菌种──庆大霉素产生菌和卡那霉素产生菌，选出的高产菌株已用于生产，提高幅度分别为２０％左右和１２．５％以上，经济效益显著。

Mutagenic effects of Streptomyces ribosidificus,Streptomyces kanamyceticus and the phage-resistant culture of Streptomyces kanamyceticus induced by N+ and C+ ion implantation with different doses have been investigated.The experimental results show that the death rates of antibiotic-producers increase with the increase of ion implantation dose, and the form mutation of the antibiotic-producers is rather obvious. After N+ ion implantation,the titer units increase by 10%-25%, 5.2%12.1% and 2.1%-12.75% for the above three strains respectively; while after C+ion implantation the titer units increase by 10%-16.9%,1.05%-3.08% and 5%-20% respectively.The selected strains of Micromonospora echimospoora and Streptomyces kanamyceticus after N+ ion implantation have been used in the factory.The increase of production is 20% and 12.5% respectively, showing marked economic benefits are obtained.