雄激素对人前列腺癌（PC—3M）裸鼠模型生长的影响及其雄激素受体和蛋白激酶C变化特性

Influences of androgen on the growth of human prostate cancer PC-3M model in nude mice and the changes of the androgen receptor levels and protein kinase C activity

采用雄性激素治疗移植人前列腺癌细胞株（ＰＣ—３Ｍ）所复制的裸鼠模型．结果表明，低剂量丙酸睾丸素（５０ｍｇ／ｋｇ体重）明显促进肿瘤生长，同时肿瘤组织中雄激素受体（ＡＲ）水平及蛋白激酶Ｃ（ＰＫＣ）活性均明显增高；而高剂量丙酸睾丸素（４００ｍｇ／ｋｇ体重）则明显抑制肿瘤生长，且肿瘤组织中ＡＲ水平及ＰＫＣ活性明显降低．显示雄激素对人前列腺癌（ＰＣ－３Ｍ）细胞株具有双相效应．讨论了这种双相效应与ＡＲ和ＰＫＣ变化的关系．

Nude mice bearing transplanted human prostate cancer cell line PC-3M were treated with male sex hormone. Results demonstrated that low dose of testosterone propionate (TP)(50mg/kg wt.)stimulated the tumor growth,and the androgen receptor(AR)levels and protein kinase C (PKC) activity were elevated in the tumor tissue.On the contrary higher dose of TP(400mg/kg wt.) inhibited the tumor growth,and the AR level and PKC activity in tumor tissue were reduced significantly.These results showed that TP has a biphasic effect on the growth of human prostate cancer PC-3M cell line. The mechanism of the biphasic effect and its relationship between AR and PKC levels are also discussed.