^（60）Coγ射线对羊草培养细胞的影响

THE EFFECT OF ￣(60)Coγ RAY ON THE CULTURE CELL OF Leymus chinensis

本文研究了羊草［Ｌｅｙｍｕｓｃｈｉｎｅｎｓｉｓ（Ｔｒｉｎ）Ｋｉｔａｇ］愈伤组织在￣（６０）Ｃｏγ射线不同剂量及不同剂量率辐照后，其染色体变异及生长和分化的情况。结果表明，３０～１００Ｃｏγ射线均引起染色体丢失、并抑制细胞的有丝分裂。染色体变异频率与愈伤组织的生长及分化的抑制与剂量及剂量率有对应关系。不同继代时间的培养细胞对γ射线的敏感度不同，以继代两天的愈伤组织最敏感。本研究对羊草作为供体的不对称体细胞杂交的研究有重要参考作用。

The chromosomal variation and the growth and differentiation of the calli of Leymus chi-nensis irradiated by ￣(60)Co-γ rays at different dose and dose rate were investigated.It wasshown that γ-rays in the range of 30～100Gy induced a lot of structural variations of chro-mosome. such as fragementation,ring formation etc.and led to chromosomal eliminationand inhibition of mitosis of the cells.The rate of chromosomal variation and the degree ofinhibition in growth and differentiation of the callus were in relation to the dose and doserate of γ- rays.The sensitivity of the cell to γ-rays is depended on the time of subculture,After two-day’s subculture the calli were most sensitive. The results provide the cytologcalevidence of the effect of Y-rays on the cultured cell of Leymus chinensis and may be valuablefor asymmetrical cell hybridization of L.chinensis , which plays a role as a donor。