摘要
应用FRTL-5大鼠甲状腺细胞研究了无氯化钠低渗透压反应液促进促甲状腺激素(TSH)刺激的甲状腺细胞合成环磷酸腺苷(cAMP)的机理。结果:无氯化钠低渗液组细胞内和细胞外液cAMP浓度均高于含氯化钠等渗液组。无氯化钠低渗液组细胞外液cAMP与总cAMP比率高于对照组。低渗液组细胞内和细胞外液cAMP浓度随反应时间延长持续升高;等渗组细胞内cAMP浓度在反应10min后达最高水平,以后无增加。结果表明,无氯化钠低渗反应液可能对提高甲状腺细胞膜cAMP的通透性,增强TSH受体后反应有一定作用。
Mechanisms of promoting the synthesis of cAMP in thyroid cells by hypotonic assay solution (NaCl-deprived) were studied with FRTL-5 thyroid cells,The intracellular and extracellular cAMP contents after stimulation with TSH were measured with both hypotonic and isotonic assay solution.In the hypotonic group the extracellular and intracellular cAMP concentrations were higher than those in the isotonic group.The ratio between the extracellular cAMP and the total cAMP in the hypotonic group was higher than that in the control.In the hypotonic group, the concentrations of the intracellular and extracellular cAMP increased steadily with the prolongation of reaction time. Under isotonic condition,the intracellular cAMP concentration reached its peak in 10 minutes,whereas it increased progressively under hypotonic condition. Therefore hapotonic assay condition increases the cAMP permeability of the thyroid cell membrance and the postreceptor reaction of TSH.
出处
《河南医科大学学报》
1995年第2期164-167,共4页
Journal of Henan Medical University