中国与美国人食管和贲门粘膜上皮中谷胱甘肽S转移酶表达的对比研究

Expression of glutathione S-transferase in esophageal and gastric cardial epithelium among subjects from China and the United States

利用免疫过氧化氢酶法，对中国人和美国人食管上皮和中国人贲门上皮谷胱甘肽Ｓ转移酶ＧＳＴ三种亚型的表达进行测定。中国人食管上皮ＧＳＴα、ＧＳＴπ和ＧＳＴμ的阳性表达率分别为５３％、８０％和２０％；贲门上皮的表达率分别为９２％、７７％和３１％。与中国人相比，美国人食管上皮三种ＧＳＴ亚型表达率均低于中国人。在贲门上皮，从正常到慢性浅表性胃炎和慢性萎缩性胃炎，ＧＳＴπ和ＧＳＴμ的阳性表达率也逐渐升高；在中国人食管，从正常上皮到基底细胞增生到间变，ＧＳＴπ和ＧＳＴα的阳性表达率逐渐升高。这些结果将有助于加深对ＧＳＴ在食管和贲门上皮中的表达及其作用的了解。

The objective of the present study is to determine the expression of GSTα, GSTπ and GSTμ in the esophageal and gastric cardial epithelium among the subjects from China and the United States with immunohistochemistry method. The immunoreactivity was observed in cytoplasm and nuclei. The positive expression rates of GSTα. GSTπ and GSTμ in the esophageal epithelium of the Chinese samples were 53%、80% and 20% respectively, while those in the gastric cardia were 92%, 77% and 31% respectively. The expression of GST in esophageal epithelium of the American samples was lower than that in the esophageal epithelium of the Chinese samples. For the gastric cardia, the positive immunostaining rates of GSTπ and GSTμ increased with the lesions progressing from normal to chronic superficial gastritis (CSG) and chronic atrophic gastritis (CAG); for the esophagus of the Chinese samples, the positive immunostaining rates of GSTπ and GSTα increased with the lesions progressing from normal to basal cell hyperplasia. These findings may shed light on the role of GST expression in the esophageal and gastric cardial carcinogenesis.