摘要
采用免疫过氧化氢酶法,分析9种P53蛋白不同抗体的特性以及P53蛋白水平分析中存在的技术问题,结果提示不同的固定方法和抗体可影响P53蛋白的测定,目前尚不能用免疫组化的方法区分出野生型和突变型P53蛋白。
With the immunoperoxidase method, the characteristics of 9 different P53 antibodies and the problems in P53 protein detection were analyzed. The results indicate that different P53 antibodies and fixation methods may affect the correct determination of P53 proteins and that the wild type and mutant type of P53 proteins can not be differentiated with the present methods of immunohistochemistry.
出处
《河南医科大学学报》
1995年第2期117-120,共4页
Journal of Henan Medical University
基金
国家自然科学基金
美国NCI联合资助