人贲门癌WAF－1基因和P53肿瘤抑制基因表达的初步研究

Expression of WAF-1 protein (P21) and P53 protein in human gastric cardial carcinoma

采用免疫组织化学方法，初步观察３４例手术切除后的贲门癌组织ＷＡＦ－１基因蛋白（Ｐ２１）的表达状况并与Ｐ５３肿瘤抑制基因蛋白的免疫组化结果进行比较。贲门癌组织Ｐ２１免疫组化阳性率为７４％；癌细胞Ｐ２１免疫反应强度与正常细胞相似。

To understand the relation between the expression of P21 and P53 in human gastric cardia adenocarcinoma, an immunohistochemical approach using anti-P21 and anti-P53 monoclonal antibodies was performed on gastric adenocarcinomas from 34 surgically -resected human specimens. Positive immunostaining for P21 was observed in 74% of the cases. The intensity of the immunoreactivity by the P21 antibody in the cancer cells was similar to that in normal cells. In the neighbouring mucosa, P21 was observed in some of the cells from the neck proliferation zone, surface mucus regenerative or proliferation epithelia,intestinal metaplasia of the gastric mucosa,and in the second to fourth layers of basal cells of squamous epithelia. Of the 22 cases with P53 accumulation,there were 5 cases with no detectable P21, 11 cases with P21 detectable in less than 12% of the cancer cells, and 6 cases with P21 detectable in more than 50% of the cancer cells. The functional relation between P21 and P53 remains to be further investigated.