人食管和贲门癌前病变和癌组织P40上皮特异性蛋白表达的研究

Studies on expresion of epithelial-specific protein of P40 in precancerous and cancerous lesions of human esophageal and gastric cardia

利用鼠单克隆抗体７Ｅ（１２）Ｈ（１２），对人食管和贲门正常粘膜上皮，癌前病变和癌组织的Ｐ４０上皮特异性蛋白表达进行比较研究。结果表明，食管和贲门上皮Ｐ４０蛋白的表达和分布明显不同。贲门柱状上皮Ｐ４０蛋白的免疫反应强度和发生率明显高于鳞状上皮（相差８倍）。食管固有腺上皮细胞也出现较强的Ｐ４０蛋白免疫反应性。在贲门组织中，Ｐ４０蛋白免疫反应６２％发生在细胞浆；２１％发生在细胞核；１７％同时发生在胞浆和胞核。而在食管，其反应全部在胞浆。随着病变的加重，食管和贲门Ｐ４０蛋白的表达均出现增高的趋势。

Comparative studies were performed on human esophageal and gastric cardia epithelia with normal, precancerous and cancerous lesions by immunohistochemistry with a mouse monoclonal antibody, 7E_(12)H_(12), developed against epithelial-specific protein of P40. The difference was observed in expression and distribution of P40 on esophageal and gastric cardia epithelia. The immunoreactivity in the gastric cardial columnar epithelium was stronger than that in the esophageal squamous epithelium. Stronger immunostaining was also observed in deep glands of esophagl. In gastric cardia biopsies, the immunoreactivity of P40 was observed more frequently in the cytoplasm (62%) than in the cell nuclei (21%). Common immunoreactivity in both cytoplasm and nuclei was observed in 17% of the cases. In esophageal biopsies, immunoreactivity was observed only in cytoplasm. A trend was observed that expression of P40 increased both in esophagus and gastric cardia with the progression of lesions. The high frequency of P40 protein level in columnar epithelia may shed light on the carcinogesis of gastric cardia.