增生过长子宫内膜和内膜腺癌雌激素、孕酮受体的检测

Assay of estrogen and progesterone receptors in endometrial hyperplasia and adenocarcinoma of endometrium

应用酶联亲和组化法检测９７例增生期、分泌期、腺瘤型增生过长子宫内膜及内膜腺癌的雌激素受体（ＥＲ）和孕酮受体（ＰＲ），并应用微波技术与常规组化染色相比较。结果经统计学处理：子宫内膜腺癌的ＥＲ、ＰＲ阳性率高于非肿瘤性子宫内膜（Ｐ＜０．０５），其与癌组织分化程度有关，但与肌层浸润深浅及绝经情况无关；增生期与分泌期宫内膜受体差异有显著性（Ｐ＜０．０５），但与增生过长子宫内膜受体阳性率无显著性差异（Ｐ＞０．０５）。提示：①ＥＲ、ＰＲ的检测为研究子宫内膜癌的发生及临床内分泌治疗提供理论依据；②微波技术是一项快速、灵敏的新方法，值得进一步推广应用。

In this study,the determination of estrogen receptor(ER)and progesterone receptor(PR) was performed on the endometrium at the proliferative and secretory stages,adenomatous hyperplasia of endometrium,and adenocarcinoma of endometrium of 97 specimens with the enzyme-linked affinity histochemistry method.And by using the technique of microwave, this was compared with regular histochemical staining. Through statistical treatment,the results showed that the positive rates of ER and PR in adenocacinoma of endometrium were significantly higher than those in the non-neoplastic endometrium (P<0. 05).They were related to the tumor differentiation, but there were no significant statistical differences in the degree of muscle infiltration and menopausal status, There were significant differences between the receptors on endometrium at the proliferative and secretory stages(P<0.05),but no significant differences in adenomatous hyperplasia of endometrium(P> 0.05). The results suggested that the discovery of ER and PR might provide theoretical basis for the genesis of adenocarcinoma and the clinical endocrine therapy.The data suggested that microwave technique might be a fast,sensitive and new method.Further popularization is needed.