突发性耳聋患者血清中人类巨细胞病毒和单纯疱疹病毒的检测

Detection of HCMV and HSV infection in sudden deafness patients

采用ＰＣＲ和ＥＬＩＳＡ方法对３０例突发性耳聋（突聋）患者血清中人类巨细胞病毒 （ＨＣＭＶ）和单纯疱疹病毒（ＨＳＶ）进行了检测。结果显示：７例（２３％）ＨＣＭＶ ＤＮＡ阳性，４例 ＨＣＭＶ抗体（ＩｇＡ或 ＩｇＭ）升高或 ＩｇＭ升高，２例 ＨＣＭＶ和 ＨＳＶ ＤＮＡ均阳性，相应抗体水平高于 正常，治疗后１月复查部分病例转阴。提示：用ＰＣＲ和ＥＬＩＳＡ方法对突聋患者病毒感染情况的检 测，对于探讨突聋的发病机制和治疗有重要意义。

The infection of HCMV and HSV were studied with PCR and ELISA in30 cases of sud- den deafness. HCMV in the peripheral blood and the antibody (IgA and IgM) in serum were monitored. The result showed that 7 (23 % of the total )sudden deafness patients were infected by HCMV, 5 (17% of the total)by HSV, and 2 by both of them. Their antibody levels were beyond the average. Since PCR technique is quick and sensitive,ELISA can monitor the con tinuous changes of antibody levels in patients. They will provide an ideal method in the prevention and treatment of virus infection. Using PCR and ELISA may further help define the role of viral etiology in sudden deafness.