碱性成纤维细胞因子改善鼓膜穿孔愈合的研究

Study on the healing of tympanic membrane perforation with basic fibroblast growth factor

在豚鼠的双侧鼓膜上制成机械性穿孔，右耳为治疗耳，每ｄ滴用碱性成纤维细胞因子 （ｂＦＧＦ）０．４８μｇ，左耳为对照耳，滴生理盐水，２１ｄ处死。病理切片显示：在穿孔愈合处，ｂＦＧＦ治疗 组的鼓膜中层厚度（４１５±３６）μｍ，对照组（１２５±３０）μｍ，两组间差异显著（Ｐ＜０．０１），治疗组鼓膜 中层成纤维细胞、胶原明显增多。提示：ｂＦＧＦ可有效改善鼓膜穿孔愈合的质量，使穿孔的鼓膜得到 最佳恢复。

Mechanical traumatic perforation was made in the tympanic membranes of both ears in guinea pigs. The right ear was the treated ear to which 0.48μg of basic fibroblast growth factor (bFGF) were dropped every day, while the left ear regarded as the control,ear was treated with saline solution. All guinea pigs were killed in 21 days. Pathologic sections showed that the thickness of the middle layer of the healed tympanic membrane was (415± 36)μm in the bFGF group,and that in the control group was (125±30)μm. There was significant difference between the two groups (P<0. 01). Fibroblast and collagen increased significantly in the tympanic membrane of the middle layer in the treated group. The results indi cated that bFGF can effectively improve the quality of the healed perforation and allow the maximal recovery of tympanic membrane perforation.