肝素对小鼠实验性内毒素血症的保护作用

THE PROTECTIVE EFFECT OF HEPARIN ON EXPERIMENTAL ENDOTOXEMIA IN THE MICE

本文采用ＬＤ＿（７５）的Ｏ＿（１１１）Ｂ＿４大肠杆菌内毒素建立小鼠实验性内毒素血症模型，观察肝素对内毒素血症的保护作用。结果，生理盐水对照组存活率为３３．３３％，肝素对小鼠内毒素血症有显著保护作用，５．５Ｕ／２０ｇ小鼠组存活率６３．６４％（Ｐ＜０．０５）；３．０Ｕ／２０ｇ小鼠组为７９．１７％（Ｐ＜０．００５）；１．５Ｕ／２０ｇ小鼠组７５．００％（Ｐ＜０．００５）。肝素注射时间对小鼠内毒素血症保护作用依次为肝素与内毒素同时注射（７９．１７％）；内毒素注入后３０ｍｉｎ（７５．００％）；内毒素注入前３０ｍｉｎ（７０．８３％）和注入前６０ｍｉｎ（５８．３３％）。注射途径依次为静脉注射（７９．１７％）、皮下注射（７７．２７％）、腹腔注射（７５．００％）、肌肉注射（７０．８３％），统计学上无明显差异。表明中小剂量肝素对小鼠内毒素血症有明显保护作用，早期使用、静脉和皮下注射疗效明显。

Using LD_(75) dose of O_(111)B_4 Eschericia coli endotoxin to attack the mice asendotoxemia model,the protective effect of heparin on endotoxemia in the mice wasobserved.The survival rate of normal saline control group was 33. 33%,In comparison withcontrol grorp,heparin had a marked protective effect on endotoxemia in the mice and thesurvival rate of 5.5 U heparin per 20 g weight of mouse group was 63. 64%(P<0.05),3.0U group was 79. 17%(P<0.005),1.5 U group was 75. 00%(P<0. 005).The protectiverates of mice were 79.17% when heparin was injected with endoxin at the same time,75. 00% in 30 min after endotoxin was injected,70. 83% and 58.33% in 30 min and 60 minrespectively before endotoxin was injected.The survival rate of mice given heparinintravenously was the highest(79. 17%),the subcutaneous was 77.27%,the peritoneal was75. 00%,the intramuscular was 70. 83%。 There was no significant difference in different timeand routes of heparin application. These results show that the medium or low dose heparinhas remarkable protective effect on endotoxemia in mice,and it is necassary to give heparin inthe early time.