Ge—132在蛋鸡体内的药代动力学

Study Pharmaco kinetics of Ge-132 in Laying Hens

给24只蛋鸡单剂量灌服Ge-132,分别于0.25～12小时采集血液、肝脏、肾脏、脾脏、卵巢和卵子,用石墨炉原子吸收分光光度法测定样品锗含量,进行药时曲线拟合和动力学参数分析。结果表明,血液中锗有2只鸡符合一级吸收单室开放模型,药代动力学参数为T_(1/2ka)0.2562±0.0302h,T_(1/2k) 3.9657±0.7075h,Tp1.1556±0.1332h,Cmax 2.1621±0.4165uσ/ml,AUC10.6164±0.1748mg/L.o;另4只鸡符合一级吸收双室开放模型,参数为T_(1/2k) 0.3996±0.2606h,T_(1/2α) 0.8513±0.1626h,T_(1/2β)4.7368±0.3652h,Tp1.0116±0.4574h,Cmax 2.6134±0.4574μg/ml,AUC13.3926±3.4651mg/Lh,在各种组织器官中均呈现一级吸收单室开放模型,其消除速率以脾脏最快,依次为脾>肝>肾>卵巢>卵子,表明了蛋鸡体内的Ge-132代谢在个体和不同器官隔室间存在很大差异。

Organic germanium compounds have been used therapcutically within research and clinical practices for almost two decades, Its metabolism and distribution have not been yet clear. Therefore the pharmacokinctics of carboxycthylgcrmanium scsquioxide(Ge-132) in laying hens was studied and discussed in this papcr.A single oral administration of 200mg Ge-132/kg BW was given to 24 laying hens. Germanium contents of wholc blood and different tissues was determined with pyrolytic graphite cuvette flamclcss AAS. The results showed that the comccntration-time curve in two laying hens was found to be of single compartment open model with first order absorption, The pharmacokinetic parameters wcrc:Yl/2ka0.2562±0.0302, Tl/2k3.9657±0.7075,Tpl.l556 ± 0.1332h,Cmax2.1627±0.4l65ug/ml, AUC10.6164±0.1748mg/L.h, The pharmacokinetic parameters of two compartment model with 4 laying hens were: Tl/2ka0.33996 ± 0.2606h, Tl/2a0.8513 ± 0.1620h, T1/2B4.7368 ± 0.3652h, Tpl.0116 ± 0.4574h, Cmax2.6134 ± 0.4574ug/ml, AUC13.3926 ± 3.465lmg/L.h.The single compartment open model with first order absorption was found in tissues.The elimination rate in spleen was faster than the other tissues, i.e. spleen >livcr >kidncy >ovarian >eggs, which indicated that metabolism of Ge-1 32 in the laying hens was significantly difference in different hens and tissues.