新城疫V_4株免疫和攻毒鸡外分泌物中病毒抗原的检测

Detection of Viral Antigen in the Vitro Secretion of NDV_4 Immunized and Challenged Chicken

利用间接夹心ELISA对新城疫V4(NDV_4)免疫鸡,免疫攻毒鸡和对照攻毒鸡口腔和泄殖腔内的病毒抗原进行了检测。结果,雏鸡免疫后第2d,口腔内病毒抗原的随机检出率为4/10,泄殖腔内随机检出率很低(0/10),持续18d以上。攻毒后第1d直到第6～8d雏鸡全部死亡时,对照鸡口腔和泄殖腔内均能查到病毒抗原。免疫鸡攻毒后全部保护,没有任何临床症状。攻毒后1d,口腔和泄殖腔内病毒抗原的随机检出率分别是8/10和6/10。第13d,泄殖腔内病毒抗原消失;而此时口腔内病毒抗原检出率仍较高(7/10),可能与鸡群内普遍存在的V4病毒有关。

The viral antigen fron the oral carity and cloaca of chickenwith NDV4 Vaccination or F48E8 challenge was detected by indirectedsandwich ELISA. The results indicated that the position rate of viralantigen from the aral cavity was 4/10,but that of from the cloaca waslower at second day post-vaccination.The viral antigen from the oralcarity and the cloaca of contral challenged chicken could be detectedat the first bay , up to dead at the sixth-eighth days post - challenge.While the immunized chicken was challenged.the protective efficacy was100%.The position rale of viral antigen from the oral cavity or cloacawas 8/10 and 6/10,respectively,but the viral antigen from the cloacawas quickly climinated at only the thirteenth post-challenge. The higherposition rate (7/10)of viral antigen from the oral carity might beralate to wadesprcad NDV4 virion in the immunized chicken.