摘要
应用感染貉细小病毒（ＲＰＶ）的７２ｈ猫肾传代细胞（Ｆ８１）制做细胞抗原片，利用超速离心结合蔗糖密度离心法提纯ＲＰＶ病毒作为免疫用抗原，制备了豚鼠抗ＲＰＶ高免血清，在酶标ＳＰＡ染色法的基础上测得免血清最佳工作范围为１６０＾Ｘ，３２０＾Ｘ，６４０＾Ｘ，ＰＰＡ的最佳工作效价为４０＾Ｘ，被检品与标准阳性血清作用最佳温度为３７℃，时间为６０ｍｉｎ，确立了酶标ＳＰＡ抑制染色法的试验程序。
In the present paper.the cell antigen slids was prepared Usingfeline Kindey cells infected by raccoon parvorius (RPV)for 72 hours.RPV,grown in feline kindey cells,was parified through the sucrose densitygradient hypervelocity centrifugation. Hypcrimmune sera against RPV Wereobtaincd fron guinea pigs which were inoculated with purified RPVcombined with Freurd adjuvant. PPA inhibition staing was established forthe detection of RPV.
出处
《黑龙江畜牧兽医》
CAS
北大核心
1995年第8期1-5,共5页
Heilongjiang Animal Science And veterinary Medicine
