摘要
应用家用微波炉及饱和硫氰酸铅溶液对35倒不同陈旧性甲醛固定石蜡包埋组织切片进行免疫组化染色,其中间叶性恶性肿瘤组织5例,流行性出血热肺、肾上腺、甲状腺组织各10例,分别使用Vimentin、Serotonin、C1同相应组织中的抗原反应,同时与常规免疫组化进行对照。结果表明:改良的免疫组化法与常规免疫组化法相比,不需要酶的消化处理,阳性染色强度大,范围广。提示,应用微波炉及金属槽液能促使陈旧性石蜡包埋组织中抗原的恢复,其染色效果明显优于常规酶液处理之方法。
cases of different long-term formalin fixed paraffin embedded tissues were stained with immunohistochemistry using domestic microwave oven and metal solution(saturated lead thiocynate),including 5 cases of mesenchymal malignant neoplasm tissues;10 cases autoptic lung,adrenal,thyroid tissues of epidemic hemorrhagic fever respectively.The antibodies such as vimentin,serotonin and C1 were used to detect the antigen of corresponding tissues in comparison with traditional immunohistochemistry.The results showed that the improved immunohistochemistry could increase positive staining intensity and scope obviously,without the treatment of enzyme digestion.It indicated that the application of microwave oven and metal solution in immunohistochemistry could retrieve the antigencity of long-term formalin fixed paraffin embedded tissues,the positive staining effects were prior to the general immunohistochemistry apparently.