摘要
研究了组织培养结合秋水仙素不同浓度和处理时间对苎麻试管苗的诱导效果，发现组织培养可以明显提高处理株的成活率，０．１％秋水仙素处理６ｄ仍有３０％左右的植株成活．首次采用不定芽技术稳定苎麻多倍体，获得了四倍体细胞达９４％的单株，并发现了三倍体和非归整倍体细胞．同时对处理过的试管苗进行了气孔鉴定和染色体检查，发现试管苗的气孔鉴定可靠性大．
Tissue culture connected with traditional methods is used to studying
ramiepolyploid. The inducing effects of colchicine of different concentration and treating time on
tubeseedlings are compared. It is found that tissue culture can increase the survial rate of
treated plantsthat 1/3 plants survives after 6 day-treatment with 1/1000 colchicine. It is the first
time thatadventitious buds would be used to stabilize ramie polyploid ; and tetraploid plants
with almosttetraploid cells, some triploid cells, aneuploid cells are ebtained. The tests of
stomate dimensionsand chromosome numbers of treated plants are carried out, which shows
that there are remarkabledifference among different ploidy plants in stomate dimension, which
can be used as a reliableindex in identifring the ploidy of tube plants.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
1995年第4期361-365,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金
关键词
苎麻
多倍体
组织培养
Boehmeria
nivea
polyploid , tissue cultures
