Percoll梯度离心法分离牛X精子及配种试验

PERCOLL DENSITY GRADIENT CENTRIFUGATION IN SEPARATINGBULL X-BEARING SPERM AND IN ANIMAL INSEMINATION IN ANIMAL

用改良Ｐｅｒｃｏｌｌ梯度柱离心分离牛精子，并将分离后富含Ｘ精子的底层精液进行配种实验。结果发现，底层精子活率正常，含Ｆ小体的Ｙ精子仅１０．２％。将其鲜精直接配种母牛１３头；冻精配种８１头。鲜精组产仔率８４．６％，冻精组为７２．８３％，与对照组比较无差异。母犊出生率，鲜精组为８１．８０％，冻精组为７１．１８％，分别比对照组提高３６．３８％与２５．７８％（Ｐ＜０．０１）。结果表明，此分离方法可提供富含Ｘ精子的精液；应用于人工配种。

eparation of bull X-and Y-bearing sperm by the Percoll density gradient centrifugationand the succession animal test with the X sperm-rich fraction were described in this paper. After centrifugation,the sperm was distributed on the interface with discontinuous Percolldensity gradient.Samples form each interface were collected and their purity of the separa-tion was examined by meams of F-body.The content of the F-body sperm in the bottom ofthe tube was only 10.2±2.6%,meanwhile,the motility of the separation sperm was nor-mal.Artificial insemination was performed in 13 heads of milk cow,using the fresh bottomX sperm-rich fraction and in 81 heads of milk cow,using the freezing bottom X sperm-richfraction. The pregnant rate was 84.6%,and 72.83%,respectively,which was the same asthe control group. The birthrate of female calf was as high as 81.%,in fresh sperm group,71.18%in freezing sperm group.an increase of 36.38%,25.78%respectively,in comparisonwith the control group.In the development milk cow enterprise,the method of Percoll densi-ty gradient centrifugation could provide X sperm without loss of its motility.