正常人血中可溶性HLA－IgG复合物的初步研究

A STUDY ON sHLA CLASS I ANTIGEN-IgG COMPLEX IN NORMAL HUMAN SEKUM AND PLASMA

用微量ＥＬＩＳＡ技术在包被有Ｗ６／３２Ｔｅｒａｓａｋｉ微量板上，利用酶标抗人β２ｍ抗体和抗人ＩｇＧ抗体测定正常人血清或血浆，并以纯化ＨＬＡⅠ类抗原、纯化人ＩｇＧ和β２ｍ等作对照。结果显示：２４份正常人血清、５１份正常人血浆中ＨＬＡⅠ类抗原与ＩｇＧ呈正相关，相关系数分别为ｒ＝０．９８５０和ｒ＝０．９６９６，而对照均为阴性。提示正常人血清或血浆中存在可溶性ＨＬＡⅠ类抗原ＩｇＧ复合物。

On non-wettable Terasaki trays coated with streptavidin-biotin-W6/32,24 sampl6S ofnormal serum and 51 normal plasma on the trays were determined byusing antibdies ofperoxidase-conjugated sheep anti-β2. microglobulin and peroxidase-conjugated sheep anti-hu-man lgG, The controls (purified HLA class I antigen, human β2 microglobulin, human IgGand 0.5%HSA-PBS)were tested. The testing results were as follows: linear correlation ex-isted between IgG and sHLA class I in serum of 24 healthy adults(correlation rate=0. 9850),and linear correlation between IgG and sHLA class I in plasma of 5l healthy adults(correlation rate=0. 9696). This implicated that soluble HLA class I antigens were presentin normal Lserum or plasma in a form of sHLA-IgG complex. This might be the reason whysome human immunoglobulin preparations are contaminated by soluble HLA class I anti-gen,The binding pattern and biological significance of the complex are not yet clear , whichneed further investigation.