抗间日疟原虫红内期单克隆抗体的研制和鉴定

PRODUCTON AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST ERYTHROCYTIC STAGE OF PLASMODIUM VIVAX

用Ｐｅｒｃｏｌｌ密度梯度离心法从志愿者血液中分离出感染间日疟原虫的红细胞。以此作为免疫原，免疫Ｂａｌｂ／ｄ小鼠，获得５株杂交瘤细胞系。融合率平均为４５％，抗体阳性率为０．６６％。用ＩＦＡｆｏＩＰＡ染色进行免疫组化分析，４Ｂ２和８Ｅ３分泌的单克隆抗体呈较强的阳性反应，与约氏疟原虫红内期无交叉反应。

With Percoll density gradient centrifugation, the erythrocytes infected P. vivax fromvolunteers were separated as immunogen and immunized Bale/c mice,in which spleen cellswere fused with mouse mveloma cells SP2/0.Monoclonal antibodies(McAb)secreted by hy-bridomas cell lines 3D5,4B2,4F11.5C7.8E3 were screened by EIJISA and cloned.In IFAand IPA staining assay,McAb 4B2 and 8E3 showed strong positive reaction with P. vivaxblood stage parasites,The cytopla2m of the parasites showed bright green fluorescencewhereas the nucleus did not.The parasites were clearly visible as dark brown bodies afterimmunoperoxidase staining. McAb 4B2 and 8E3 did not show cross-reaction with the erythrocytic stages of P. yoelii.