摘要
通过ELIB技术对曼氏裂头蚴可溶性抗原的免疫学特性进行分析,并用ELISA法对特异性抗体作了检测。结果表明:裂头蚴抗原内有5种组分被感染兔血清识别,其中低分子量组分是主要的血清学抗原;裂头蚴感染可使宿主产生高水平的抗体反应;用ELISA法诊断裂头蚴感染简便可行。
Teh
immunological haracteristics of Sparganum mansoni soluble antigen andits crossreaction with
several other parasites were analysed by means of ELIB and ELISA. The re-sults of ELIB
showed that there were five reactive bands with serum mixture of two Spar-ganum mansoni
infected rabbits,and the 26.5~30KD proteins were the main serologicalantigens。Sparganum
mansoni antigens had cross reaction against several other parasites,a-mong those the
Cysticercosis cellulosae was the most distinct one. The results of ELISAshowed that the titer of
specific antibody reached l:1280,but for most cross reactions,thetiter of them was lower than 1
:80.This indicated when the detecting serum was diluted to1:100,the result of ELISA was an
important reference for diagnosing Sparganum mansoniinfection in human.
出处
《湖南医科大学学报》
CSCD
1995年第3期206-208,共3页
Bulletin of Hunan Medical University
关键词
多节绦虫亚纲
裂头蚴属
免疫印迹法
抗原
诊断
cestods
Sparganum
enzyme-linked immunosorbent
assay
im-munoblotting
antigens
antibodies
diagnostc use