摘要
测定24例用ACD保养液于4℃下不同贮存期全血红细胞的Ca2+ATP酶活性。结果表明,贮存2周内酶活性无明显变化,3周后开始下降,至第4周酶活性完全消失。红细胞氧耗量检测结果显示,CPD组的保存效果似较ACD组为佳。提示红细胞膜Ca2+-ATP酶活性可考虑作为血库对贮存血质控的参考指标之一;而氧耗率则有助于对保存效果的检查。
In this study,blood
samples were collected from 24 healthy donors and their wholeblood were stored in
acid-citrate-dextrose(ACD) anticoagulant separately at 4℃. The mem-brane Ca2+ ATPase
activities in the stored RBC were measured following 7,14,21 and 28days after the
storage,Whole blood from 3 donors were also selected and divided into twoportions which
have been stored in citrate-phosphate-dextrose(CPD) and ACD anticoagu-lants respectively,with
the oxygen consumption rate of erythrocytes determined within 10,20 and 35 days after the
storage.The results showed that Ca2+ ATPase activitv did notchange obviously until 21 day
after the storage and disappeared completely on the 28th day。From the evaluation of oxygen
consumption rate, it was found that the efficiency of bloodpreservation in CPD group seemed
better than ACD group. Our data suggest that membraneCa2+ ATPase activity may be
considered as one of the qualitative criteria for blood preservation and Oxygen consumption
rate as an auxillary criterion.
出处
《湖南医科大学学报》
CSCD
1995年第4期341-343,共3页
Bulletin of Hunan Medical University
关键词
血液保存
红细胞
钙
氧耗率
腺苷三磷酸酶
blood preseration
red blood cells
Ca2+ transporting
ATPase
oxy-gen consumption rate