氧自由基对体外小鼠脾细胞产生IL－2的影响及其机制初探

EFFECTS AND SOME MECHANISMS OF OXYGEN FREE RADICALS ON IL-2 PRODUCTION FROM MURINE SPLENOCYTES IN VITRO

观察了氧自由基在体外对Ｂａｌｂ／ｃ小鼠脾细胞产生ＩＬ－２的影响，并就其影响机制进行了初步研究。结果发现，１０－３ｍｏｌ／Ｌ的过氧化氢抑制小鼠脾细胞ＩＬ－２的产生，而１０－７～１０－４ｍｏｌ／ＬＨ２Ｏ２以及黄嘌呤氧化酶系统（黄嘌呤氧化酶１０－１Ｕ／ｍｌ，次黄嘌呤５×１０－５ｍｏｌ／Ｌ）产生的超氧阴离子对小鼠脾细胞产生ＩＬ－２具促进作用。其作用机制可能是通过影响细胞内ｃＡＭＰ的浓度从而影响淋巴细胞的活化。

Exposing splenocytes of Balb/c mice to 10-7～10-4mol/L H2O2 for 1hour resulted inmarkedly increasing production of IL-2 induced by ConA,while the exposure lasted 3 hours,the improving effects disappeared;IL-2 produced from these cells was suppressed by 10-3mol/L H2O2. A possible mechanism that H2O2 regulated IL-2 production from murine spleno-cytes may be mediated through a cAMP pathway. In given conditions an generating sys-tem(xanthine oxidase plus hypoxanthine) enhanced IL-2 production from murine spleno-Cytes.