热休克反应对大鼠油酸肺保护作用的机制研究

THE MECHANISM OF PROTECTIVE O0F HEAT SHOCK RESPONSE ON OLEIC ACID-INDUCED LUNG INJURY IN RATS

未研究发现，经热休克处理的大鼠注油酸后，肺组织损伤明显减轻，Ｗｅｓｔｅｒｎ印迹分析显示热休克使大鼠肺组织中ＨＳＰ７０含量明显增多；Ｎｏｒｔｈｅｒｎ印迹分析证实此乃由于ＨＳＰ７０ｍＲＮＡ转录增加所致。说明热休克处理使肺组织ＨＳＰ７０基因表达增强；提示热休克反应对大鼠油酸肺的保护作用与肺组织细胞中ＨＳＰ７０基因表达增强及与ＨＳＰ７０增多有关。

ur previous work had found that heat shock offered protection from oleic acid inducedlung injury in rats, but the mechanism of protection afforded by heat shock remains un-known,This study tested the hypothesis that induction of heat shock proteins by heat shockcontributes to organ protecive efffect of heat shock in oleic acid-induced acute lung injury.Heat shocked animals were warmed to 42 C for 1 5 min, 24h before intravenous injection ofoleic acid.Western blot analysis showed that after heat shock, HSP70 in pulmonary tissuewas elevated,and northern blot analysis indicated that the increased concentration of HSP70was accompanied by increase in the corresponding HSP70 mRNA.Lung histopathologicexamination showed that there were cellular interstitial infiltration with alveolar edema andcellular exudate in unheated control animals, and by contrast,there was well-preserved pul-monary architecture with minimal cellular interstitial infiltrate and alveolar exudate in heatshocked animals. These data indicated that heat shock pretreatment associated with the in-duction of HSP70 gene expression and HSP70 synthesis in lung tissue attenuates oleic acid-induced lung injury in rats.