IBV　S1基因不对称PCR的研究

STUDY ON ASYMMETRIC POLYMERASE CHAIN REACTION OF THE INFECTIOUS BRONCHITIS S1 GENE

ＤＮＡ模板为ＩＢＶ广东地方株Ｄ４１经病毒ＲＮＡ提取后反转录而获得；两引物为ＩＢｖＢｅａｕｄｅｔｔｅ株Ｓ１基因两侧的对应序列；跨幅为１．７ｋｂ。当限制性引物（Ｏｌｉｇｏ３’）终浓度为０．８ｍｇ／Ｌ、两引物比例为１：１０时，即得到不对称ＰＣＲ的预计单链和双链ＤＮＡ产物。此研究结果及不对称ＰＣＲ反应条件为国内首次报道。

A DNA template was got by reverse transcription of the IBV Guangdong localstrain D41 genome RNA.Two synthetic primers corresponding to sequences on bothflanks of the IBV Beaudette strain spikeⅠ(S1)gene,facilitated PCR amplification of1700-base sequence.When the final concentrition of the limiting primer (OLIGO3’)was 0.8 mg/L and the ratio of two primers 1:10,we got expected single-anddouble-stranded DNA products of asymmetric PCR,This is the first report onasymmetric PCR of IBV Slgene in China.