葡萄试管苗土支撑培养带坨移栽研究

A Study on Transplanting with Lump Grape Plantlets in vitro Cultured in Soil Supporting Mdium

以土作为培养基支撑物，离体培养葡萄试管苗双节茎段，４０天后即达到适于炼苗标准，成苗率９５．８％。带坨移入营养钵，在不喷雾、不覆膜、空气湿度低至４５％条件下，成活率达９６．７％。成活的试管苗炼苗５４天后移入大田，移栽成活率为１００％，其中特级苗占８７．５％，一级苗占１２．５％。试验证明，土支撑培养带坨移栽技术是简化试管苗移栽程序、降低成本的有效措施。

Stem sections with two nodes from in vitro cultured grape plantlets were cultured in soil supporting medium for 40 days, 95.8% of the stem sections produced new plantlets which subsequently underwent a four-week-long acclimatization. The ptantlets were then transplanted with lump into soil, and 96.7% of them survived under the conditions of no spraying of water,no plastic film covering, and a low relative air humidity of 45%. 54 days later, the survived plantlets were further transported into field, and in autumn they all formed young plants in which the super-grade plants and first-grade plants accounted for 87.5% and 12.5%respectively. The results showed that the techniques described above can effectively simplify the transplant procedure and reduce the cost of the plantlets derived from in vitro culture.