观赏羽衣甘蓝的离体繁殖

In vitro Propagation of Ornamental Kale (Brassica oleracea var. acephala f. tricolor Hort.)

以花蕾为外植体，离体繁殖羽衣甘蓝（Ｂｒａｓｓｉｃａｏｌｅｒａｃｅａｖａｒ．ａｃｅｐｈａｌａｆ．ｔｒｉｃｏｌｏｒＨｏｒｔ．）８个杂种一代材料。培养基为（ｍｇ／Ｌ）：ＭＳ＋ＢＡ１～２＋ＮＡＡ０．１～０．２＋ＩＡＡ０．５＋（或不加）ＧＡ３０．３＋３％蔗糖。平均１８％～６７％的接种花蕾产生不定芽；约有３０％的不定芽发育成完整植物。培养物的严重褐化和再生植物的普遍玻璃质化是影响植株成活的主要因素。培养基中加入活性炭和青霉素，并给以强光照射，可提高植株成活率。

Flower buds of eight F1 cultivars of ornamental kale (Brassica oleracea var. acephala f. tricolor Hort.) were in ditro propagated as explants. The medium containing MS+BA 1-2 mg/L +IAA 0. 5mg/L +NAA 0. 1-0. 2mg/L +GA3 0. 3mg/L(or free) +3% sucrose was used for the induction of adventitious buds. The adventitious buds were induced at the rate of 18-67% of the cultured flower buds. At the rate of 30%, the regenerated adventitious buds developed into complete phatlets. The browning of cultures and the glassy of regenerated ptantlets severely effected the survival rate of the plants. Addition of active carbon and penicillin (Gpotassium) into the medium under strong lighting(25000 Lux) could raise the survival rate of the phatlets.