低温对杂交一代黄瓜幼苗生理特性的影响

Effect of Chilling on Some Physiological Characteristics of F_1 Hybrid Cucumber Seedling

对四个黄瓜亲本及其杂交一代（Ｆ１）的生理特性试验研究表明，Ｆ１的相对电导值与双亲的相对电导值呈正相关关系。ＭＤＡ含量在低温处理４８ｈ后上升，７２ｈ后下降，较耐低温亲本的Ｆ１的ＭＤＡ含量低于双亲平均值，不耐低温亲本的Ｆ１的ＭＤＡ含量高于双亲的平均值；低温处理７２ｈ后ＣＡＴ酶活性下降，ＰＯＤ酶活性上升；ＰＯＤ酶同工酶分析表明，低温处理后酶带数增加，耐低温的Ｆ１酶带增加较多。

Four cucumber parents and their two F1 hybrids were studied on the inheritance of some characteristics under chilling stress. The results indicated that the relative electrical conduct of F1 hybrids was positively correlative to that of their parents in the whole process of chilling.Content of MDA firstly increased after 48 hours and decreased after 72 hours of treatment.Contents of MDA in F1 hybrids which were relatively tolerant to chilling were lower than the mean value of MDA in their Parents, but were higher in chilling-intolerant ones. After a chilling treatment of over 72 hours, the activity of CAT enzymes decreased, but the activity of POD enzymes increased. The analysis of POD isozymogram showed that the number of the enzyme bands increased after chilling treatment, and that the increase of enzyme bands in chilling-tolerant F1 hybrids was greater.