禽霍乱克隆89弱毒菌苗安全性稳定性及应用效果

Safty, Stability, and Efficiency Tests on an Attenuated Fowl Cholera Bacterine Of Strain Clone89

研究结果显示，用５×１０９到１０×１０９禽霍乱克隆８９弱毒活菌苗肌注５３日龄到１２月龄蛋鸡、肉鸡是安全的。将菌苗回归本动物鸡体１３代次，逐渐加大传代剂量，菌苗毒力不但不增强，反而越来越弱，从第７代开始临床无反应，从１１代开始，肌注部位无炎症反应，说明克隆８９菌苗毒力安全稳定，不会出现毒力增强的返祖现象，通过１１０万羽鸡中试，在本病流行季节前一次肌注免疫，即有效控制病情。对暴发本病的鸡、鸭群，早期紧急接种，２天稳定疫情，３至５天死亡停止。

The attenuated fowl cholera vaccine of strain Clone89 was shown to be safe when administered intramuscularly into layer and broilers of 53 days-12 months old with the doses of 5×109 and 10×109 CFU bacteria respectively. No inflammatory reaction was observed at the injection sites 5 days after administration of 3×107 CFU bacteria. The strain did not renew its virulence, on the contrary, it became less and less virulent after 13 continuous back-passages in chickens with raised doses. No clinical signs from the 7th passage and no local inflammatory reaction from 11th passage were observed, which indicated that no atavism in virulence appears when the bacterine is put into use for Vaccination. One point one million of chickens were vaccinated in field trials. Before the epidemic seasons of the disease, one shot vaccination could control the disease. The clinical sighs calmed down in 2 days and deaths ceased in 3-5 days after early emergency vaccination of infected cholera flocks.