用光敏生物素标记鸡传染性支气管炎病毒cDNA探针及其应用的研究

STUCIES ON THE PREPARATION AND APPLICATION OF AVIAN INFECTIOUS BRONCHITIS VIRUS cDNA PROBE

应用光敏生物素标记鸡传染性支气管炎病毒ｃＤＮＡ，制备核酸探针、经斑点杂交法和碱性磷酸酶显色后，探针同以ｃＤＮＡ为模板合成的ＰＣＲ产物及其重组子ｐＳＸＩＢＶＳ１均呈现强阳性的蓝色斑点，而与正常尿囊液、新城疫病毒和鸡败血霉形体无杂交。一个初诊为ＩＢ感染的田间病料与探针杂交阳性结果相符合。试验表明该ｃＤＮＡ探针是敏感和特异的检测方法。

In this study,an Avian Infectious Bronchitis Virus(IBV)cDNA probe labelled withphotobiotin was prepared.Using blot hybridization and the AV-AP staining system,the pro be showed strongly positive results when hybridized with a PCR product andits recombinant pSXIBVS1,but no hybridization signal was exhibited in other speci-mens such as NDV,MG and normal allantoic fluid. A field sample suspected to befrom an IB infected birds gave result which corresponded to the positive hybridizationreactions.The study showed that the photobiotin-labelled IBV cDNA probe was ahighly sensitive and specific method of detection。