应用亲和层析制备萝卜叶溶菌酶

PURIFICATION OF A LYSOZYME FROM Raphanus sativus LEAVES BY AFFINITY CHROMATOGRAPHY

应用脱氨几丁质亲和层析直接从萝卜叶粗提液中制备溶菌酶，所得精酶比活力为４２１６２Ｕ／ｍｇ蛋白，活力回收为６５％，得率为１Ｏ．６μｇ／ｇ鲜叶，纯化倍数为７７．４倍。效果优于应用几丁质、ＣＭ－几丁质的纯化效果。

A lysozyme from Raphanus sativus leaves was purified by affinity chromatographyon a crab deaminated chitin column.The specific activity of the purified lysozymewas increased by 77.4 folds and reached 42162 U/mg protein,the recovery of totalactivity was 6 5 %,and the yield of the purified lysozyme from the leaves was10.6μg/g fresh weight。