化疗药物对体外培养人舌癌细胞的即时杀伤效应

The Immediate Killing Effect of Commonly Used Chemotherapeatic Drugs on Human's Tongue Cancer Cell in Vitro

以人舌鳞癌ＴＣａ８１１３细胞作为术中脱落癌细胞的模型，观察盐酸氮芥、平阳霉素、氨甲喋呤、顺氯氨铂、５氟尿嘧啶对癌细胞作用５ｍｉｎ时的杀伤效应。实验结果表明：１．化疗药物短时间内对癌细胞的杀伤数及有丝分裂抑制有明显差异，２．除５氟尿嘧啶外，癌细胞再次体外培养时均不能增殖，结果提示临床上应用氮芥、平阳霉素、氨甲喋呤及顺氯氨铂作为冲洗液，能有效地抑制术中脱落的癌细胞。

Abstract The authors used the human's tongue cell line(Tca 8113)as an experimental modle in vitro to study the cell immediate killed effect of commonly used anticancer drugs after exposure 5 minutes.The results were as follows:1.The numbers of living cell reduced significantly,so did the indeces of mitoses.2.All the drugs except 5-Fu could utlerly kill the cells.The results indicated that clinical irrigation of surgical wound with HN2,PYM and DDP solution could effectively prevent tumor recurrence after surgery.