摘要
用反相高效液相色谱法对生脉散制剂中人参皂甙类成分进行分离,并测定处方中人参皂甙Rgl含量。用InertsilODS-2柱,乙腈-0.05%磷酸(19:81)为流动相,203nm为检测波长。方法准确、精密、灵敏、分离良好,可用于多种样品分析。
In this paper we sueccssfully estabalished a RP-HPLC method for the separation of Ginsenosides in Shengmai San preparation and quantitatively determined Ginsenoside Rgl in preparation,The HPLC conditions were Inertsil ODS-2 column,CH3CN-0.05%H3PO4(19: 81)as mobile phase,detection at 203 nm.The curve of Y=170446.8x+23864.1,r=0.9999 within 25100ng~200.8ng,method recovery was 98.63%,CV%=2.4%(n=9),within day precision CV%=0.21%,Inter day precision CV%=0.48%(n=9),detective limit 100ng (S/N=5:1),lowest detective concentration 10ug/ml(S/N=5:1).The established method was proved to be accurate, precise,sensitive and had a very good separation. It can be empolye to the analysis of various samples.
出处
《华西药学杂志》
CAS
CSCD
北大核心
1995年第1期21-24,共4页
West China Journal of Pharmaceutical Sciences