双波长分光光度法测定复方氢化可的松搽剂的含量

DETERMINATION COMPLEX HYDROCORTISONE LINIMENT BY BIWAVELENGTH SPECTROPHOTOMETRIC METHOD

采用双波长分光光度法测定复方氢化可的松搽剂１号的含量。测定波长为２４７ｎｍ，参比波长为２６５ｎｍ。在６～１４μｇ／ｍｌ范围内，△Ａ值与氢化可的松（Ｈｙｄｒｏｃｏｒｔｉｓｏｎｅ，简称ＨＣ）的浓度呈良好的线性关系。回归方程为Ｃ＝１．８８８１△Ａ－０．００４９５９，ｒ＝０．９９９９（ｎ＝５）．平均回收率为１００．４％，ＲＳＤ＝０．６１％（ｎ＝６）。该法简便快速，结果准确。

The determination of complex Hydrocortisone liniment I by biwavelength spectro-photometric method was reported. λ1=247nm,λ2=265nm.The good linear relationship between △A and concentration was obtained within the range of 6～14μg/ml(C=1.8881 △A-0.004959,r=0.9999).The average recovery was 100.4%,RSD=0.61%(n=5).This method is simple,rapid and accurate.