我国主要致病钩端螺旋体DNA与OmpL1基因同源性的研究

Homology Study of Leptospires in China with the OmpL1 Gene

用￣（３２）Ｐ标记的ＯｍｐＬ１基因片段与我国１８株钩体进行Ｓｏｕｔｈｅｒｎ杂交分析，此片段与非致病钩体ＰａｔｏｃＩ株、伊利尼细丝体３０５５株无杂交信号；问号钩体中，除爪哇群、塔拉索夫群、曼耗群和明尼群的４株钩体外，黄疸出血群、犬群、拜伦群、致热群、秋季群、澳洲群、波摩那群、流感伤寒群、七日热群、巴达维亚群、赛罗群各群钩体参考株ＤＮＡ均有杂交信号。

Southern hybridization analysis withthe OmpL 1 gene was performed. The results of prob-ing EcoRI-restricted genomic DNA from 1 8 st rains inChina showed that the homology fragments of OmpL1gene were presented in 1 2 pathogenic Leptospirastrains:sero-group Icterohaemorrhagiae(2 strains),Canicola,Ballum,Pyrogenes,Autumnalis,Australis,Pomona,Grip-potyphosa,Hebdomadis,Bataviae,andSejroe,but they were not presented in the non-pathogenic Leptospira biflexa strain Patoc I,Leptone-ma illini strain 3055 and pathogenic I,eptospiraSerogroup Javanica,Tarassovi,Manhao and Mini.