胎儿股骨骺板和干骺端胰岛素样生长因子－Ⅰ分布的研究

Localization of Instlin-Like Growth Factor I in the Upper End of Human Fetal Femur

采用硬组织切片技术和ＡＢＣ免疫组织化学技术，对５例１６～２０周胎儿股骨骺板、干骺瑞进行胰岛素样生长因子－Ｉ（简称ＩＧＦ－Ｉ）的定位研究。观察到骺板储备区、增生区和肥大区均有ＩＧＦ－Ｉ免疫反应阳住的软骨细胞；干骺端存在一定数量的ＩＧＦ－Ｉ阳性成骨细胞，部分类骨质和骨基质也有阳性反应物沉积，少数破骨细胞也呈ＩＧＦ－Ｉ免疫阳性反应。作者同时讨论了在硬组织切片上进行免疫组化染色的可能性和ＩＧＦ－Ｉ在骨组织内的作用。

The localization of insulin-likegrowth factor I in the upper end of the human fetalfemur was studied by immunohistochemistry on theundecalcifled bone sections.Certain numbers of. chon-drocytes in geriminal, proliferating and hypertrophiczones of the epiphysial growth plates , some os-teoblasts and a few osteoclasts were immunoreactive tothe antibody to IGF-I. The end-product of the DABreaction was deposited in the osteoid and bone matrixof metaphysis. Also presented is a discussion on thelocal effects of IGF-I on the chondrocytes, osteoblastsand osteoclasts.