人体皮肤枪弹创局部组织中5－HT的免疫组化研究

Immunohistochemical Study of 5-HT on Gunshot Wound of Human Skin

用免疫组化ＰＡＰ法观察人体皮肤生前枪弹创标本４０例、死后３０例损伤局部５－ＨＴ的变化，发现生前枪弹创局部５－ＨＴ主要分布于创缘、皮肤乳头层、皮下疏松结缔组织及皮肤附件周围结缔组织内，皮肤网状层结缔组织内５－ＨＴ分布较少；死后８分钟内形成的枪弹创改变与生前相同。免疫组化ＰＡＰ法亦证实人体皮肤肥大细胞颗粒内不含５－ＨＴ，表明用免疫组化ＰＡＰ法观察损伤局部５－ＨＴ的变化对生前死后损伤的鉴别有重要价值，并为前人生化方法测定结果提供了形态学依据。甲苯胺蓝染色，发现生前枪弹刨距创缘０～４．０ｍｍ区域内肥大细胞脱颗粒率均＞５０％，与死后枪弹创标本比较有显著差异，故可认为肥大细胞脱颗粒率变化可为生前死后损伤鉴别依据之一。

Fourty antemortem and 30 post-mortem gunshot wound samples of human skin werestudied by immunohistochemical method,The 5-HTwas seen mainly in wound edge, papillary layer,hy-podermis and surrounding tissue of all antemortemgunshot wounds. The 5-HT was also discovered onthe postmortem gunshot wounds which occured within 8 minutes after death.The resuIt showed that im-munohistochemical staining of 5-HT be useful for di-agnosing the antemortem gunshot wound.It also de-monstrated that themast cells of human do not contah5-HT. We also studied the rate of mast cell degranula-tion of gunshot wounds of human skin by toluidineblue staining, The rate of mast cell degranulation ofantemortem gunshot wounds increased(50%), and itwas higher than that of postmortem gunshot wounds,suggesting that the rising of degranulation rate is asign of antemortem injury.