摘要
利用细胞膜电容监测技术和显微镜形态观察的方法,研究了全细胞记录模式对肥大细胞分泌反应的影响。结果表明,机械力与电场力对细胞分泌反应有一定的抑制作用,胞浆内离子和活性小分子的冲洗流失则是影响分泌反应的根本原因。最后提出了全细胞记录模式对肥大细胞分泌反应的作用机制。
With the monitoring of cell membrane capacitance and by observation of the microscopicmorphology,the effects of the whole cell recording mode on the secretory response of mastcells are studied.The results show that both the mechanical force and electric field force in-hibit the secretory response to a certain extent and the wash-out of intracellular ions andsmall active molecules is the essential cause.The mechanism of the effects of the whole cellrecording mode on the secretory response of the mast cell is given.
出处
《华中理工大学学报》
CSCD
北大核心
1995年第11期80-84,共5页
Journal of Huazhong University of Science and Technology
基金
国家教委博士点基金
关键词
膜片钳
全细胞记录
肥大细胞
分泌
patch clamp
whole cell recording
mast cell
secretion.,