小麦原生质体培养与植株再生的研究

PROTOPLAST CULTURE AND WHEAT PLANTLET REGENERATION(TRITICUM AESTIVUML.)

用缩短继代间隔的方法，从小麦３５８１６幼穗愈伤组织中筛选到由小颗粒组成的松脆型胚性愈伤组织，并建立了胚性细胞悬浮系。从中分离的原生质体培养在经高压灭菌的改良ＭＳ或改良Ｎ６培养基中，得到了大量的再生愈伤组织和胚状体。经直接分化或增殖后分化均得到再生植株。生长迅速的再生愈伤组织具有较高的植株再生能力；将再生细胞团增殖后分化能明显提高植株再生率和再生植株数。随着悬浮培养时间的增加，原生质体培养中直接胚胎发生途径将减少。

This paper reprots on the somatic embryogenesis and callus formation from protoplasts isolated from embryogenic cell suspension with high regenerability of wheat(Triticum aestivum L.cv.35816).The division frequency was up to 8% on modified MS or N6 medium by autoclaving.Plantlets were regenerated by direct differentiation and differentiation after subcultures.Calli or embryoids which grew fast had higher plant regenreability.Higher regenerating frequency and more plantlets were obtained by differentiation after subculture.With an increase in the time of suspension culture,the frequency of embryogenesis of protoplast cultures declined.