昆虫细胞低血清和无血清培养基的研究

STUDIES ON SERUM-REDUCED AND SERUM-FREE MEDIA FOR INSECT CELL CULTURE

昆虫细胞低血清和无血清培养基均以ＴＣ－１００和ＴＣ－１９９－ＭＫ培养基混合液为基础液，补加微量元素，适量的维生素、甘油、肌苷等成份．研究结果表明：两种培养基均能支持小菜蛾细胞（Ｐｘ细胞）的生长和苜蓿银纹夜蛾核型多角体病毒（ＡｃＭＮＰＶ）在其中复制．

Two new serum-reduced and serum-free media (SRM and SFM) for insect cell culture were described in this paper. Both of them were based on the combination of TC-199-MK and TC-100 media,and supplemanted with trace elements,vitamins,glycerol and inosine. In addition,the SRM was supplemented with 0. 4%lactalbumin hydrolysate, yeast extract, lipid concentration and 2% new--born calfserum,while the SFM contained egg yolk emulsion. The studed results showed atboth the SRM and SFM were able to supporthe grouth of plutella xylostella (px)cell and the replication of Autographa californica multiple nuclear polyhedrosisvirus (AcMNPV) in Px cells.