辣椒（Capsicum　annuum　L．）下胚轴离体培养的研究

PLANT REGENERATION FRON HYPOCOTYL EXPLANTS OF CHILLI PEPPER (CAPSICUM ANNUUM) IN VITRO

将辣椒无菌苗下胚轴切段接种在ＭＳ培养基（附加不同的植物激素）上，诱导产生愈伤组织．最适的诱导培养基为ＭＳ＋２ｍｇ／ＬＢＡ，０．５ｍｇ／ＬＩＡＡ，在此培养基上产生的愈伤组织具有较强的分化能力，将愈伤组织转移到分化培养基，光照培养，芽分化率可达２５．０％·不定芽在含、１ｍｇ／ＬＧＡ３和２ｍｇ／ＬＢＡ的ＭＳ培养基上伸长成苗，再移入生根培养基上诱导生根，长成完整植株．

in the present report, we studied conditions of plant regeneration from hypocotyl explants of chilli pepper (Capsicum annuum). The results obtained in hypocotyl culture showed that the MS medium containing 2 mg/L BA and 0. 5 mg/L IAA was favourable for forming compact and nodular calli. Shoot buds were regenerated on the surface of the calli with a frequency of 25. 0% when the calli were transfered on MS medium with 4 mg/L BA and 0. 5 mg/L IAA under condition of light (25℃, 2 000 lux, 12 h/d). Shoots elongated on the medium with 2 mg/L BA and 1 mg/L GA3, and subsequently rooted on MS medium with 0. 2 mg/L ICA. The plants were transplanted into pots and growing well in field.