500升絮凝颗粒酵母连续发酵悬浮床生物反应器流体力学行为

HYDRODYNAMIC BEHAVIOR OF 500 L SUSPENDED-BED BIOREACTOR SUITABLE FOR CONTINUOUS ETHANOL FERMENTATION USING FLOCS

利用某些酵母细胞的自身强絮凝能力形成颗粒,以固定化细胞法实现酒精连续发酵,近年来引起了人们的极大关注。与通常的各种载体固定化细胞法相比,它具有方法简单、过程经济的突出优点,而且反应器中无载体充填,可以获得更高的菌体浓度,进而强化反应器的设备生产强度指标。然而,这种由细胞自身絮凝形成的颗粒有很大的特殊性。一方面,它的形状、结构极不规则,颗粒的大小在一定的条件下呈特定的分布。另一方面,颗粒间具有粘连性,且自身强度弱,不耐流体剪切,在培养与发酵过程中要求与基质呈均匀的悬浮状态。因而,开发研制适宜的生物反应器,并解决其工程放大问题成为这一技术能否工业化的关键。

Using the floes self - flocculated from Schizosaccharomyces pombe, the hydrddy-namic behavior in the suspended -bed bioreactor with a working volume 500L was studied. It was discovered that the washing -out and shearing breakage for floes caused by CO2 turbulence could not be neglected when this bioreactor was scaled -up. Further, a design mechanism for an industrial - scale suspended - bed bioreactor was proposed