用差热分析法研究铜（Ⅱ）离子对人血清白蛋白热变性的影响

A DLA STUDY TO THE EFFECT OF COPPER(Ⅱ)ION ON THE THERMAL DENATURATION OF HUMAN SERUM ALBUMIN

用差热分析法研究铜（Ⅱ）离子对人血清白蛋白热变性的影响曾成鸣，丁敏，孙凡（重庆医科大学检验系，重庆，６３００４６）（西南农业大学基础部，重庆，６３０７１６）关键词：白蛋白，铜，热变性，差热分析热分析技术已被广泛用于生物物质在各种条件下的热力学性质的研...

In this paper,the thermal denaturation of human serum albumin in phosphate buffer solutionwith different concentrations of copper (Ⅱ)ion were studied by differential thermal analysis (DTA) method.The results showed that the denaturational temperature(Td)and the denaturational heat(Qd)increased with an increasing order of copper concentrations, which suggested that copperion could inhibit the thermal denaturation of human serum albumin.Another endothermal peak before thermal denaturation of the protein was also observed in the thermograph ,which the peak temperature (Tn)increased and the peak area decreased with the in crease of copper concentration.