蛋白核小球藻与DEP的相互作用

Study on the Interaction between Chlorella pyrenoidosa Chick and Diethyl Phthalate.

运用评价化学品对藻类毒性的标准实验方法，得出邻苯二甲酸二乙酯（ＤＥＰ）抑制蛋白核小球藻生长的９６ｈ－ＥＣ５０为８０ｍｇ／Ｌ。实验结果表明，蛋白核小球藻对ＤＥＰ有明显的富集与降解作用，富集量和浓缩系数（ＢＣＦ）都在１２ｈ达到最大，分别为９．８ｍｇ／ｇ和２０５。此后富集量随时间的延长而逐渐降低，９６ｈ降到１．８ｍｇ／ｇ。ＢＣＦ在７２ｈ内也随时间的延长而逐渐减小，并在７２ｈ降到最小８０，７２ｈ以后又随时间的延长而升高，９６ｈ增加到９１。蛋白核小球藻日平均降解７．３ｍｇ／ＬＤＥＰ，日平均降解率１４．６％。用动力学方程（－ｄｅ／＆＝ＫＮ，）拟合降解过程，计算值与实测值的平均相对偏差为４．０％。

The standard method of algal bioassay for evaluting toxicity of toxic chemicals was applied and the 96h--EC50 of diethyl phthalate (DEP)inhibitillg the growth of Chlorella pyrenoidosa was calculated as 80mg/L.It was found that C. pyrenoidosa was able to accumulate and biodegrade DEP.Accumulated amount of DEP and biological concentration factors(BCF) reached maximum values(9.83mg/g,205) at 12 h. As time went on afterward,accumulated amount declined gradually with time and reached a minimum value (1.8 mg/g) at 96 h,and BCF also decreased gradually with time before 72 h and reached minimum (80) at 72 h,but after 72 h,BCF increased gradually with time and reached 91 at 96h. Average biodegraded amount of DEP per day was 7.3 mg/L and average biodegradation rate per day was 14. 6 %.If using kinetic equation of --de/dt = K N T to fit the biodegradation process,the average relative deviation between calculated values and observed values was 4.0%.