高效薄层析进行农药吸附态光解的研究

Method for Testing the Photodegradability of Herbicides Adsorbed on HPTLC Plates

研究了绿麦隆和消草醚２种除草剂在硅胶Ｇ高效薄层上的光稳定性，提出应用高效薄层析技术作为农药吸附态光解的一种试验方法。在高效薄层上直接点样，同一薄层上包括照光、黑暗对照和定量曲线样品，原始剂量４００—８００ｎｇ·１×５ｍｍ斑点－１，照光后直接展开和扫描定量测定，最低检测量可达２０ｎｇ·斑点－１。本方法无须对样品进行提取、净化等处理，具有快速高效、重复性好，且可直观光解产物等优点。

A rapid and effective method to detect the photodegradability of herbicides in an adsorption state was developed with a high performance thin layer chromatography(HPTLC) technique. Two herbicides, chlortoluron and fluorodifen, were applied directly on a silica gel 60 F254 high performance thin layer plate(10X20cm) by Linomat Ⅳin a dosage of 400-800 ng per slit, then irradiated under natural sunlight, and then the HPTLC plates were developed and measured with a DESAGA 60 Scanner. The photolytical dynamics of chlortoluron and fluorodifen, the optimal dosage and other test conditions were also described in this paper.