模拟N、P污水对水稻幼苗过氧化物酶和超氧化物歧化酶的影响

EFFECTS OF MIMIC WASTEWATER CONTAINING N,P ON PEROXIDASE AND SUPEROXIDE DISMUTASE IN RICE SEEDLINGS

以水稻为材料，研究了不同浓度的模拟Ｎ、Ｐ污水对幼苗过氧化物酶（ＰＯＤ，ＥＣ１．１１．１．７）和超氧化物歧化酶（ＳＯｏＤ，ＥＣ１．１５．１．１）及其同工酶等的影响，结果表明：在总氮浓度（Ｎｒ）为０─９０ｍｇ／Ｌ和总磷浓度（Ｐ＿Ｔ）为０─９ｍｇ／Ｌ范围内，ＰＯＤ与Ｎ、Ｐ浓度呈正相关，Ｎ＿Ｔ超过９０ｍｇ／Ｌ和Ｐ＿Ｔ超过９ｍｇ／Ｌ这一阈值后呈下降趋势，ＮＴ在０─１２０ｍｇ／Ｌ和Ｐ＿Ｔ在０─１２ｍｇ／Ｌ范围内，ＳＯＤ，丙二醛（ＭＤＡ）与Ｎ、Ｐ浓度呈显著正相关；Ｎ、Ｐ能诱导产生新的同工酶带，苗高、可溶性蛋白分别与Ｎ、Ｐ浓度呈显著负相关．

EHects of mimic wastewater on activities andisoenzyme of peroxdase(EC1.11.1.7POD)and superoxide dismutase(SOD, EC 1.15.1.1)were studied at various concentra-tions of nitrogen and phosphorus, The results showed that when N_T<90 mg/L and P_T<9mg/L, there were significant positive correlations between POD activity and the concentra-tion of N and P,while POD activity declined when N_T > 9 0 mg/L and P_T > 9 mg/L. SODactivity and content of malondialdehyde(MDA)were positively correlated to the concentra-tion of N and P significant.In addition,effects of N and P on heights of rice seedlings andcontents of soluble protein in their leaves were studied.The results indicated that heightsnegatively correlated to the concentration of N and P,and so did the contents of soluble pro- tein.