卒中型高血压大鼠心肌线粒体Ca^（2＋），Mg^（2＋）—ATPase及膜流动

Study on the Ca^(2+), Mg^(2+)-ATPase and Membrane Fluidity of Myocardial Mitochondria from SHRsp Rat

本文比较了卒中型自发往高血压鼠（ＳＨＲｓｐ）和京都Ｗｉｓｔａｒ正常血压鼠（ＷＫＹ）心肌线粒体Ｃａ￣（２＋），Ｍｇ￣（２＋）－ＡＴＰａｓｅ活力及膜流动性。用定磷法测得ＷＫＹ和ＳＨＲｓｐ的Ｃａ￣（２＋），Ｍｇ￣（２＋）－ＡＴＰａｓｅ活力（２５℃）分别为０．２８７±０．０１６及０．２１８±０．０１７μｍｏｌ／（ｍｉｎ．ｍｇ）（－ｘ±Ｓｘ）。ＳＨＲｓｐ心肌线粒体Ｃａ￣（２＋），Ｍｇ￣（２＋）－ＡＴＰａｓｅ活力降低２４．７％，两组比较有显著差异（Ｐ＜０．０１，ｎ＝９）．以ＤＰＨ标记心肌线粒体膜，测得ＷＫＹ和ＳＨＲｓｐ的荧光偏振值分别为０．１８７±０．００３及０．１８１±０．００３（Ｐ＞０．０５，ｎ＝６）。

o investigate the role of myoeardial calcium metabolism in proccessingof hypertension and hypertrophy,Ca￣(2+),Mg￣(2+)-ATPase activity and membranefluidity of myocardial mitochondria were analysed from stroke-prone sponta-neously hypertensive rats(SHRap)and normotensive wistar kyoto rats (WKY).The results showed that Ca￣(2+),Mg￣(2+)-ATPase activities of WKY and SHRspwere 0.287±0.016 and 0.218±0.017μmol/( min,mg),respectively with signifi-cant difference ( P<0.01,n=9),The fluorescence polar1zation, which reflectsthe membrane fluidity, of WDY and SHRsp were 0.187±0.003 and 0.181±0.003,respectively(P>0. 05,n=6).