铜锌超氧化物歧化酶在风湿性疾病患者滑膜中的变化

CuZn-SOD Changes in the Synovia of the Patients with Rheumatic Diseases

通过关节镜技术采取膝关节滑膜３８例，将组织标本进行冷冻切片４～６μｍ厚，放－２０℃贮存备用。应用免疫组化法测定滑膜中ＣｎＺｎ－ＳＯＤ的沉积，其中类风湿关节炎（ＲＡ）１６例；反应性关节炎（ＲｅＡ）１０例，骨性关节炎（ＯＡ）７例，非滑膜炎（Ｎｏｎ－Ｓ）５例。结果表明：１６例ＲＡ患者（均为活动性ＲＡ）滑腹中，１１例几乎无ＣｕＺｎ－ＳＯＤ沉积，其余５例散在沉积，１０例ＲｅＡ患者滑膜中偶见（＋／－），散在（＋），局灶（＋＋）性ＣｎＺｎ－ＳＯＤ沉积分别有３例，４例，３例；而１２例ＯＡ与Ｎｏｎ－Ｓ患者滑膜（非滑膜炎病变）中，１０例均为局灶性或弥漫性ＣｎＺｎ－ＳＯＤ沉积。此外，我们还发现这样的现象：在滑膜中，越是炎性细胞浸润和积聚的部位，ＣｎＺｎ－ＳＯＤ沉积就越少，尤其在ＲＡ滑膜的淋巴滤泡中，几乎没有ＣｕＺｎ－ＳＯＤ的沉积，此处氧自由基非常活跃，其对滑膜的损伤不断造成软骨和骨的破坏，这或许是ＲＡ关节腔破坏机制的一个侧面。

8 cases of synovia were adopted with arthroscopy technique and the tis-sue samples were cryo-sectioned with 4～6 μm thickness,in-20℃ store for use.We used immuno-histochemical method to measure the deposition of synovia in 16cases of RA(active RA).10 cases of reactive arthritis(ReA),7 cases of osteo-arthritis(OA),5 cases of Non-synovitis(Non-S).The results showed that in the synovia of 16 RA patients,almost no CuZn-SOD deposition in 11 cases,sparse deposition in other 5 cases,in the synoviaof 10 ReA pstients accidetal deposition in 3 cases,sparse deposition in 4cases, local deposition in 3 cases nevertheless,in the synovia of 12 OA andNon-S patients(no obvious synovia damage),local and homogenous CuZu-SODdeposites in 10 cases.Otherwise,we found such phenomenon;less CuZn-SODdeposition in the place of synovia where inflammatory cells infiltrate andaccumulate, especially in lympha follicle of RA synovia CuZn-SOD hatdly de-posied,where oxy-zadicals are very active,this damages the synovia, furthe-more destroys cartilage and bone.It may be one of the RA pathogenesis of thejoint cavity.